Protein glycosylation
	1. Assembly of the precursor oligosaccharide… IMPORTANT POINTS: – Assembly takes place on the carrier lipid dolichol, anchored in the ER membane. – A pyrophosphate bridge joins the 1st sugar to the dolichol. – Sugars are added singly and sequentially. – After the two N-acetylglucos- amines are added, the assembly flips from the cytosolic side to the ER lumen. – Nine mannose and three glucose molecules are added, totaling 14 sugars.