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| • | Y2H used to
identify potential cGKIa binding proteins. |
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| – | 2.5 x
106 clones from human activated T cell library |
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| – | Clone AL9 encoded
the COOH terminal 181 amino acids of myosin |
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| binding subunit
of myosin phosphatase. MBS is a 130
kD regulatory |
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| subunit of PP1M
that confers the specificity of PP1 for MLC and is the |
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| site on PP1M that
is regulated by rho kinase. |
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| – | The COOH terminal
181 amino acids of MBS includes a leucine zipper |
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| domain. |
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| Surks et al, Science 1999
286:1583 |